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Analysis of methane biodegradation by Methylosinus trichosporium OB3b BJM
Rodrigues,Andréa dos Santos; Valdman,Belkis; Salgado,Andréa Medeiros.
The microbial oxidation of methane in the atmosphere is performed by methanotrophic bacteria that use methane as a unique source of carbon and energy. The objective of this work consisted of the investigation of the best conditions of methane biodegradation by methanotrophic bacteria Methylosinus trichosporium OB3b that oxidize it to carbon dioxide, and the use of these microorganisms in monitoring methods for methane. The results showed that M. trichosporium OB3b was capable to degrade methane in a more effective way with an initial microorganism concentration of 0.0700 g.L-1, temperature of 30ºC, pH 6.5 and using 1.79 mmol of methane. In these same conditions, there was no bacterial growth when 2.69 mmol of methane was used. The specific rate of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Methane; Biodegradation; Methanotrophic bacteria; Methylosinus trichosporium OB3b.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200017
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Tracing carbon assimilation in endosymbiotic deep-sea hydrothermal vent Mytilid fatty acids by C-13-fingerprinting ArchiMer
Riou, V.; Bouillon, S.; Serrao Santos, R.; Dehairs, F.; Colaco, A..
Bathymodiolus azoricus mussels thrive at Mid-Atlantic Ridge hydrothermal vents, where part of their energy requirements are met via an endosymbiotic association with chemolithotrophic and methanotrophic bacteria. In an effort to describe phenotypic characteristics of the two bacterial endosymbionts and to assess their ability to assimilate CO2, CH4 and multi-carbon compounds, we performed experiments in aquaria using C-13-labeled NaHCO3 (in the presence of H2S), CH4 or amino-acids and traced the incorporation of C-13 into total and phospholipid fatty acids (tFA and PLFA, respectively). 14:0; 15:0; 16:0; 16:1(n - 7) c+t; 18:1(n - 13) c+t and (n - 7) c+t; 20:1(n - 7); 20:2(n - 9,15); 18:3(n - 7) and (n - 5,10,13) PLFA were labeled in the presence of...
Tipo: Text Palavras-chave: Mid atlantic ridge; Mussel bathymodiolus azoricus; Methanotrophic bacteria; Marine invertebrates; Diversity; Organic compounds; Lipid composition; Dual symbiosis; Methane; Biomarkers.
Ano: 2010 URL: https://archimer.ifremer.fr/doc/00207/31868/30275.pdf
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